Simpsonichthys margaritatus is a species of killifish from the family Rivulidae.Costa, W.J.E.M., 2012. Simpsonichthys margaritatus, a new seasonal miniature killifish from the upper Paraná River basin, central Brazilian Cerrado (Cyprinodontiformes: Rivulidae). Ichthyol. Explor. Freshwat. 22(4):313-318. It is found in the Verde River floodplains, of the upper Paraná River basin in central Brazil.

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