Mary Gambale (born December 18, 1988) is an American former professional tennis player.

Gambale grew up in Winchester, Massachusetts and was coached by her father Bob. She played Junior Fed Cup tennis for the United States and was later used as a hitting partner for the senior team. After winning the USTA Girls 18s National Championships as a 16-year-old in 2005, Gambale received a wildcard into the main draw of that year's US Open, where she was beaten in the first round by Dally Randriantefy.

ITF finals $75,000 tournaments $25,000 tournaments $10,000 tournaments Singles: 1 (0–1) Outcome No. Date Tournament Surface Opponent ScoreRunner-up 1. ITF Raleigh, United States Clay Olga Lazarchuk 3–6, 1–6

Doubles: 1 (1–1) Outcome No. Date Tournament Surface Partner Opponents ScoreWinner 1. ITF Hammond, United States Hard Kelley Hyndman Christina Fusano Ahsha Rolle 2–6, 6–3, 7–5Runner-up 2. ITF Southlake, United States Hard Elizabeth Lumpkin Beatrice Capra Rebecca Marino 6–3, 4–6, [6–10]

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