The Charles University Rector election, 2021 was held on 22 October 2021. The incumbent Rector Tomáš Zima was ineligible to run for third term. Milena Králíčková was elected first female rector of the university.

Voting Milena Králíčková Michal Stehlík 55 14 69 members of academic senate voted. Králíčková received 55 votes to Stehlík's 14 votes.

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