1990 Italian Open – Women's singles

Monica Seles defeated Martina Navratilova in the final, 6–1, 6–1 to win the women's singles tennis title at the 1990 Italian Open. After dropping 4 games in her first set of the tournament, Seles would only drop 10 games during her last nine sets, dropping a total of just 14 games in her five matches.

Gabriela Sabatini was the two-time defending champion, but lost in the semifinals to Navratilova.

Seeds The first eight seeds received a bye to the second round.