Mathesius is a Czech surname. Notable people with the surname include:
Bohumil Mathesius (1888–1952), Czech poet, translator, publicist and literary scientist Johannes Mathesius (1504–1565), German-Bohemian minister and Lutheran reformer Johannes Mathesius, the Younger (1544, Jáchymov – 1607), Bohemian-German physician:de:Johannes Mathesius der Jüngere Paul Mathesius (1548, Jáchymov – 1584), Bohemian-German Lutheran theologian:de:Paul Mathesius Vilém Mathesius (1882–1945), Czech linguist
Mathesius is also a surname of a Finnish family and may refer to:
Johan Mathesius (1709–1765), pastor and politician of the Caps party Per Niklas Mathesius (1711–1772), pastor and politician of the Caps party, brother of Johan Mathesius
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