The Massacre at Matanzas Inlet was the mass killing of French Huguenots by Spanish Royal Army troops near the Matanzas Inlet in 1565, under orders from King Philip II to Pedro Menéndez de Avilés, the adelantado of Spanish Florida (La Florida).

The Spanish Crown in the 16th century laid claim to a vast area that included what is now the state of Florida, along with much of what is now the southeastern United States, on the strength of several Spanish expeditions made in the first half of the 1500s, including those of Ponce de Leon and Hernando de Soto. However, Spanish attempts to establish a lasting presence in La Florida failed until September 1565, when Menéndez founded St. Augustine about 30 miles south of the newly established French settlement at Fort Caroline on the St. Johns River. Menéndez did not know that the French had already arrived in the area, and upon discovering the existence of Fort Caroline, he aggressively moved to expel those whom he considered heretics, pirates, and invaders. He marched with troops overland and attacked the Huguenot settlement, sparing only women, children, Catholic males, and a few skilled craftsmen.

When the French Huguenot leader, Jean Ribault, learned of the Spanish presence nearby, he also decided on a swift assault and sailed south from Fort Caroline with most of his troops to search for the Spanish settlement. His ships were struck by a storm (probably a tropical storm) and most of the French force was lost at sea, leaving Ribault and several hundred survivors in two groups shipwrecked with limited food and supplies: one group about 15 miles south of the Spanish colony, and Ribault's group much farther southward at Cape Canaveral. Meanwhile, Menéndez marched north, overwhelmed the remaining defenders of Fort Caroline, massacred most of the French Protestants in the town, and left an occupying force in the rechristened fort, now called "San Mateo" by the Spanish. Upon returning to St. Augustine, he received news that Ribault and his troops were stranded to the south. Menéndez quickly moved to attack and massacred the French force of two separate parties on the shore of what became known as the Matanzas River.

History Wreck of Ribault's ships On 28 September 1565, a group of local Timucua brought to St. Augustine information that a number of Frenchmen had been cast ashore on an island six leagues (about 25 kilometres or 16 miles) from St. Augustine, where they were trapped by the river (the Matanzas), which they could not cross. These proved to be the crews of two more ships of the French fleet which had left Fort Caroline on 10 September. Failing to find the Spaniards at sea, Captain Jean Ribault had not dared to land and attack St. Augustine, and so had resolved to return to Fort Caroline. When his vessels were caught in the same storm previously mentioned, the ships dispersed, and two of them wrecked along the shore between Matanzas Inlet and Mosquito Inlet. Part of the crews had been drowned in attempting to land, the Indians had captured fifty of them alive and had killed others, so that out of four hundred there remained only one hundred and forty. Following along the shore in the direction of Fort Caroline, the easiest and most natural course to pursue, the survivors had soon found their further advance barred by the inlet, and by the lagoon or "river" to the west of them.

Negotiations between first party of French survivors and the Spanish On receipt of this news Menéndez sent Diego Flores in advance with forty soldiers to reconnoitre the French position; he himself with the chaplain, some officers, and twenty soldiers rejoined Flores at about midnight, and pushed forward to the side of the inlet opposite their encampment. The following morning, having concealed his men in the thicket, Menéndez dressed himself in a French costume with a cape over his shoulder, and, carrying a short lance in his hand, went out and showed himself on the river bank, accompanied by one of the French prisoners, in order to convince the castaways by his boldness that he was well supported. The Frenchmen soon observed him, and one of their number swam over to where he was standing. Throwing himself at his feet the Frenchman explained who they were and begged the Admiral to grant him and his comrades a safe conduct to Fort Caroline, as they were not at war with Spaniards.

"I answered him that we had taken their fort and killed all the people in it," wrote Menéndez to Philip II, "because they had built it there without Your Majesty's permission, and were disseminating the Lutheran religion in these, Your Majesty's provinces. And that I, as Captain-General of these provinces, was waging a war of fire and blood against all who came to settle these parts and plant in them their evil Lutheran sect; for I was come at Your Majesty's command to plant the Gospel in these parts to enlighten the natives in those things which the Holy Mother Church of Rome teaches and believes, for the salvation of their souls. For this reason I would not grant them a safe passage, but would sooner follow them by sea and land until I had taken their lives."

The Frenchman returned to his companions and related his interview. A party of five, consisting of four gentlemen and a captain, was next sent over to find what terms they could get from Menéndez, who received them as before, with his soldiers still in ambush, and himself attended by only ten persons. After he had convinced them of the capture of Fort Caroline by showing some of the spoil he had taken, and some prisoners he had spared, the spokesman of the company asked for a ship and sailors with which to return to France. Menéndez replied that he would willingly have given them one had they been Catholics, and had he any vessels left; but that his own ships had sailed with artillery for Fort San Mateo and with the captured women and children for Santo Domingo, and a third was retained to carry dispatches to Spain.

Neither would he yield to a request that their lives be spared until the arrival of a ship that could carry them back to their country. To all of their requests he replied with a demand to surrender their arms and place themselves at his mercy, so that he could do "as Our Lord may command me." The gentlemen carried back to their comrades the terms he had proposed, and two hours later Ribault's lieutenant returned and offered to surrender their arms and to give him five thousand ducats if he would spare their lives. Menéndez replied that the sum was large enough for a poor soldier such as he, but when generosity and mercy were to be shown they should be actuated by no such self-interest. Again the envoy returned to his companions, and in half an hour came their acceptance of the ambiguous conditions.

Massacre of the first French party Both of Menéndez's biographers give a much more detailed account of the events, evidently taken from a common source. The Frenchmen first sent over in a boat their banners, their arquebuses and pistols, swords and targets, and some helmets and breast-pieces. Then twenty Spaniards crossed in the boat and brought the now unarmed Frenchmen over the lagoon in parties of ten. They were subjected to no ill treatment as they were ferried over, the Spaniards not wishing to arouse any suspicions among those who had not yet crossed. Menéndez himself withdrew some distance from the shore to the rear of a sand dune, where he was concealed from the view of the prisoners who were crossing in the boat.

In companies of ten the Frenchmen were conducted to him behind the sand dune and out of sight of their companions, and to each party he addressed the same request: "Gentlemen, I have but a few soldiers with me, and you are many, and it would be an easy matter for you to overpower us and avenge yourselves upon us for your people which we killed in the fort; for this reason it is necessary that you should march to my camp four leagues from here with your hands tied behind your backs."Norman 1968, pp. 153–154 The Frenchmen consented, for they were now unarmed and could offer no further resistance, as their hands were bound behind them with cords of the arquebuses and with the matches of the soldiers, probably taken from the very arms they had surrendered.

Then Mendoza, the chaplain, asked Menéndez to spare the lives of those who should prove to be "Christians." Ten Roman Catholics were found, who, but for the intercession of the priest, would have been killed along with the Huguenots. These were sent by boat to St Augustine. The remainder confessed that they were Protestants. They were given something to eat and drink, and then ordered to set out on the march.

At the distance of a gunshot from the dune behind which these preparations were in progress, Menéndez had drawn a line in the sand with his spear, across the path they were to follow. Then he ordered the captain of the vanguard which escorted the prisoners that on reaching the place indicated by the line he was to cut off the heads of all of them; he also commanded the captain of the rearguard to do the same. It was Saturday, 29 September, the feast of St. Michael; the sun had already set when the Frenchmen reached the mark drawn in the sand near the banks of the lagoon, and the orders of the Spanish admiral were executed. That same night Menéndez returned to St. Augustine, which he reached at dawn.

On 10 October the news reached the garrison at St. Augustine that eight days after its capture Fort San Mateo had burned down, with the loss of all the provisions which were stored there. It was accidentally set on fire by the candle of a mixed-race servant of one of the captains. Menéndez promptly sent food from his own store to San Mateo.

Massacre of Ribault and the second French party Within an hour of Menéndez receiving this alarming report some Indians brought word that Jean Ribault with two hundred men was in the neighborhood of the place where the two French ships had been wrecked. They were said to be suffering greatly, for Ribault's flagship La Trinité had broken to pieces farther down the shore, and their provisions had all been lost. They had been reduced to living on roots and grasses and to drinking the impure water collected in the holes and pools along their route. Like the first party, their only hope lay in a return to Fort Caroline. Le Challeux wrote that they had saved a small boat from the wreck; this they caulked with their shirts, and thirteen of the company had set out for Fort Caroline in search of assistance, and had not returned. As Ribault and his companions made their way northward in the direction of the fort, they eventually found themselves in the same predicament as the previous party, cut off by Matanzas Inlet and river from the mainland, and unable to cross.

On receipt of the news Menéndez repeated the tactics of his previous exploit, and sent a party of soldiers by land, following them that same day in two boats with additional troops, one hundred and fifty in all. He reached his destination on the shore of the Matanzas River at night, and the following morning, 11 October, discovered the French across the water where they had constructed a raft with which to attempt a crossing.