The 40th Assembly district of Wisconsin is one of 99 districts in the Wisconsin State Assembly. Located in central Wisconsin, the district comprises parts of northern Columbia County and eastern and southern Sauk County. It includes the cities of Baraboo, Portage, Prairie du Sac, and Sauk City, and the villages of Merrimac, Pardeeville, Plain, Spring Green, and West Baraboo. The district also contains Devil's Lake State Park and Natural Bridge State Park. The district is represented by Democrat Karen DeSanto, since January 2025.

The 40th Assembly district is located within Wisconsin's 14th Senate district, along with the 41st and 42nd Assembly districts.

List of past representatives +List of representatives to the Wisconsin State Assembly from the 40th districtMemberPartyResidenceCounties representedTerm startTerm endRef.District created Francis R. Byers Rep. Marion Outagamie, Shawano, Waupaca January 1, 1973 January 3, 1983 Earl Gilson Dem. River Falls Pierce, St. Croix January 3, 1983 Francis R. Byers Rep. Marion Outagamie, Waupaca January 2, 1989 William Lorge Rep. Deer Creek January 2, 1989 January 4, 1999 Jean Hundertmark Rep. Clintonville January 4, 1999 January 1, 2007 Rep. Waupaca Waupaca, Waushara January 1, 2007 January 6, 2025 Outagamie, Waupaca, WausharaKaren DeSanto Dem.BarabooColumbia, SaukJanuary 6, 2025Current

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