The women's 1000 meter at the 2016 KNSB Dutch Single Distance Championships took place in Heerenveen at the Thialf ice skating rink on Sunday 29 December 2015. Though this tournament was held in 2015, it was part of the speed skating season 2015–2016. There were 22 participants.
Title holder was Marrit Leenstra.
Result Rank Skater Time Jorien ter Mors 1:15.17 Marrit Leenstra 1:16.27 Ireen Wüst 1:16.294 Sanneke de Neeling 1:16.595 Margot Boer 1:16.626 Anice Das 1:17.657 Janine Smit 1:17.668 Roxanne van Hemert 1:17.689 Annouk van der Weijden 1:17.8810 Linda de Vries 1:18.1411 Suzanne Schulting 1:18.3612 Letitia de Jong 1:18.4113 Bo van der Werff 1:18.9314 Rosa Pater 1:19.0115 Lotte van Beek 1:19.1516 Annette Gerritsen 1:19.2917 Sanne van der Schaar 1:19.5018 Melissa Wijfje 1:19.7319 Isabelle van Elst 1:19.8120 Floor van den Brandt 1:19.9221 Julia Berentschot 1:19.9822 Moniek Klijnstra 2:01.41– Antoinette de Jong WDR WDR = Withdrew
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