Ashburner is a surname, and may refer to:

Charles Albert Ashburner (1854–1889), American geologist Lesley Ashburner (1883–1950), American athlete Lionel Robert Ashburner (1827–1907), American colonial administrator Michael Ashburner (1942–2023), English biologist Walter Ashburner (1864–1936), American-born British classical and legal scholar

See also Ashburner family