Justiniano is both a surname and a masculine given name. Notable people with the name include:
People with the surname Alberto Justiniano, American theatre director Gonzalo Justiniano (born 1955), Chilean theatre director Leonel Justiniano (born 1992), Bolivian footballer Pedro Justiniano (born 2000), Portuguese footballer Raúl Justiniano (born 1977), Bolivian footballer
People with the given name Justiniano Asunción (1816–1901), Filipino painter Justiniano Borgoño (1836–1921), Peruvian soldier and politician Justiniano Borja (1912–1964), Filipino politician Justiniano González Betancourt (born 1936), Mexican politician Justiniano Montano (1905–2005), Filipino politician