Hvoslef is a surname. Notable people with the surname include:
Agnes Hvoslef (1883–1970), Norwegian operatic soprano Anna Hvoslef (1866–1954), Norwegian journalist, conservative politician and feminist Einar Hvoslef (1876–1931), Norwegian sailor Ketil Hvoslef (born 1939), Norwegian composer Waldemar Hvoslef (1825–1906), Norwegian Lutheran bishop