Penola may refer to the following:
Places Antarctica Penola Island Penola Strait Australia Penola, South Australia, a town and locality Penola Conservation Park, a protected area in South Australia Hundred of Penola, a cadastral unit in South Australia United States Penola, Virginia, an unincorporated community in Virginia
Ships Penola, a sailing ship used for the British Graham Land expedition SS Penola, a steamship
Other Penola (fly), a genus of flies in the family Sphaeroceridae Penola Catholic College, a secondary school in Victoria, Australia