John Charlesworth may refer to:
John Charlesworth (American football) (1902–1962), American football player John Charlesworth (abolitionist) (1782–1864), English abolitionist and Anglican clergyman John Charlesworth (politician) (1815–1880), British colliery owner and Member of Parliament John Kaye Charlesworth (1889–1972), British geologist and academic author