Cyrtinae is a subfamily of the Acroceridae (small-headed flies). Their larvae are endoparasites of araneomorph spiders in the subgroup Entelegynae.

Genera The subfamily includes twelve extant genera and two extinct: Asopsebius Nartshuk, 1982 †Cyrtinella Gillung & Winterton, 2017 Cyrtus Latreille, 1796 Hadrogaster Schlinger, 1972 Holops Philippi, 1865 Meruia Sabrosky, 1950 Nipponcyrtus Schlinger, 1972 Opsebius Costa, 1856 Paracyrtus Schlinger, 1972 Sabroskya Schlinger, 1960 Subcyrtus Brunetti, 1926 Turbopsebius Schlinger, 1972 †Villalites Hennig, 1966 Villalus Cole, 1918

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