Pachysentis is a genus in Acanthocephala (thorny-headed or spiny-headed worms) that parasitize primates and carnivorans. The eleven species are distributed across Africa, the Middle East, and the Americas. Pachysentis species attach themselves to the inner lining of the gastrointestinal tract of their hosts using their hook-covered proboscis. Their life cycle includes an egg stage found in host feces, a cystacanth (larval) stage in an intermediate host which is usually an insect, and an adult stage where cystacanths mature in the intestines of the host. This genus appears identical to the closely related Oncicola apart from a greater number of hooks on the proboscis. There are eleven species assigned to this genus, although P. septemserialis is of uncertain taxonomic status. The female worms range from long and wide in P. lauroi to long and wide in P. dollfusi. Virtually all of the length is the trunk, with a short proboscis. There is pronounced sexual dimorphism in this species as females are around twice the size of the males. Infestation of P. canicola in the Sao Miguel island fox caused inflammation, degradation, and perforation of the intestines and death of the intestinal tissues, but no infestations in humans have been reported.
Taxonomy The genus Pachysentis was established with three species by Anton Meyer in 1931, with P. canicola designated as the type species. The genus underwent a significant expansion in 1972 when parasitologist Gerald D. Schmidt reclassified seven species into Pachysentis including six formerly belonging to Prosthenorchis and one from Oncicola. The status of P. septemserialis remains contentious; inconsistencies between the original 1950 description and the surviving paratype led Gomes et al. in 2019 to suggest it may be a synonym of P. lenti.
Phylogenetic analysis has been conducted on one of the eleven species in the genus, P. canicola. Sequences of the molecular marker 18S rDNA gene were compared to the same sequences in related species and it was concluded that P. canicola samples formed a well-supported group with other species in the family Oligacanthorhynchidae. Phylogenetic analyses have also been conducted on Oncicola, a genus morphologically nearly identical to Pachysentis apart from the number of hooks on the proboscis, and have also placed it in the family Oligacanthorhynchidae.
Description Number of proboscis hooksBarbs in hooksP. angolensis 42 No barbsP. canicola 72 No barbsP. dollfusi 48 BarbsP. ehrenbergi 102BarbsP. gethi 42 No barbsP. lauroi 48BarbsP. lenti 48 No barbsP. procumbens 90No barbsP. procyonis 42No barbsP. rugosus42 No barbs
Pachysentis looks identical to the closely related Oncicola apart from the number of hooks on the proboscis. Species of Oncicola have 36 or fewer hooks whereas species of Pachysentis have more. The probosces of Pachysentis species are not quite spherical and contain 42 to 102 hooks arranged into 12 longitudinal rows of 3 to 12 hooks each. Depending on the species, the rows may be regularly or irregularly alternating and straight or crooked. Hooks have tips with or without barbs, and the larger hooks with complex heads (manubria) and roots with the remaining spines being rootless. The trunk is around twice as long as wide with the anterior half usually wider than the posterior half. The testes are in tandem with at least one located before the middle of the worm. There are eight cement glands compactly arranged each with a single giant nucleus used to temporarily close the posterior end of the female after copulation. The eggs have a sculptured outer membrane. Species can be distinguished based on the number and arrangements of proboscis hooks, whether these hooks are barbed, the arrangement of the cement glands, host, and the length of lemnisci (bundles of sensory nerve fibers). Female measurements (mm) Measurements P. angolensisP. canicolaP. dollfusiP. ehrenbergiP. gethiP. lauroiP. lentiP. procumbensP. procyonisP. rugosusLength of proboscis0.55-0.630.57-0.80?0.80.5830.680.630.550.6970.564Width of proboscis0.70–0.820.57–0.85?0.90.7940.760.6640.550.7160.694Length of proboscis receptacle1.52?1.31.071.161.321.21.371.24Width of proboscis receptacle????0.4980.47??0.5310.481Length of trunk34–4820–265026–2915–2512.0720–25625-3532Width of trunk4.8–5.55-11461.5–31.622–2.51.252–33Length of lemnisci5.8–674.3–6.673.484.453.15?3.644.64Width of lemnisci???0.8??????Uterine bell?3.15–8.15??5.561.191.41?4.645.86Size of eggs0.09 × 0.0430.07 × 0.0450.08 × 0.050.07 × 0.050.084 × 0.0540.073 × 0.045??0.071 × 0.042?Male measurements (mm)Length of proboscis0.55-0.630.57-0.80?0.80.5830.680.630.550.6970.564Width of proboscis0.70–0.820.57–0.85?0.90.7940.760.6640.550.7160.694Length of proboscis receptacle1.52?1.31.071.161.321.21.371.24Width of proboscis receptacle????0.4980.47??0.5310.481Length of trunk17-2315-28502510–15 × 1.0–2.59.6315–20620-3025Width of trunk3.5–44-8441.0–2.51.911.0–2.51.252–33.5Length of lemnisci5.8–674.3–6.673.484.453.15?3.644.64Width of lemnisci???0.8??????Size of anterior testis2–3 × 0.92?31.40 × 0.5811.15 × 0.481.76 × 0.51?3.01 × 1.241.57 × 0.697Size of posterior testis2–4.3 × 1.02?31.40 × 0.5811.27 × 0.551.82 × 0.547?3.15 × 1.071.69 × 0.664Dimension of group of cement glands33?71.540.86 × 0.562.98?3.562.02Ejaculatory duct length2.3???4.641.42??3.531.68
Species The genus Pachysentis contains eleven species, although P. septemserialis is of uncertain taxonomic status.
Pachysentis angolensis (Golvan, 1957)
P. angolensis was found infesting the side-striped jackal (Canis adustus). The proboscis has a total of 42 hooks without barbs in 12 regularly alternating rows of three and four hooks (six rows of each). The eight cement glands are organized in pairs. This species is named after Angola, the country where it was first discovered.
Pachysentis canicola Meyer, 1931
P. canicola was found infesting the red fox (Vulpes vulpes) in Bushehr, Bushehr Province, Iran, the golden jackal (Canis aureus) also in Iran, captive maned wolves (Chrysocyon brachyurus) in a zoo in Texas, and the striped skunk (Mephitis mephitis) found nearby. An invasive P. canicola is also responsible for the decline in the indigenous Sao Miguel island fox (Urocyon littoralis) population of Sao Miguel Island detected in 69% of 40 the necropsied foxes. It was also found infesting the western diamondback rattlesnake (Crotalus atrox), a Paratenic hosts (hosts where parasites infest but do not undergo larval development or sexual reproduction). It is the type species of the genus Pachysentis. The species name canicola is derived from the Latin word for canine which is the type of creature (family Canidae) that this species infests.
Pachysentis dollfusi (Machado-Filho, 1950)
P. dollfusi was found infesting the intestines of the common brown lemur (Eulemur fulvus) in a Brazilian zoo but originally from the island of Madagascar. It is thus unknown if the worm originates from Brazil or Madagascar. The proboscis has 48 barbed hooks arranged into six rows of four hooks each followed by another six rows of four hooks each. The cement glands are in uniform pairs. It is synonymous with Prosthenorchis spirula Travassos 1917. It is named after the parasitologist Robert-Philippe Dollfus.
Pachysentis ehrenbergi Meyer, 1931
P. ehrenbergi was found infesting the red fox (Vulpes vulpes) in Egypt and in an paratenic hosts (hosts where parasites infest but do not undergo larval development or sexual reproduction), the Egyptian cobra (Naja haje). The proboscis is armed with 102 barbed hooks arranged into six rows of nine each followed by six rows of eight hooks each. P. ehrenbergi was reported infesting the body cavity of 5.4% of a sample of African five-lined skinks (Trachylepis quinquetaeniata) in the Qena Governorate of Egypt, however the measurements and morphological description do not match either the original description by Meyer in 1931 or the reexamination of original specimens by Gomes and colleagues in 2019. Juvenile P. ehrenbergi have been reported infesting the long-eared hedgehog (Hemiechinus auritus) in the Faran Oasis, South Sinai, Egypt. Juvenile trunk length is reported to be 3.22–4.16 mm by 0.87–1.04 mm, the proboscis length to be 0.42–0.60 mm in length by 0.45–0.68 mm in width, the proboscis sheath to be 0.79–1.0 mm by 0.37–0.52 mm (whereas the measurements given by Meyer in the original 1931 description is larger, at 1.3 mm in length and the proboscis measuring 0.8 mm in length by 0.9 mm in width). The anterior proboscis hooks in the juveniles are reported to be large, from 0.078–0.086 mm long, and the posterior hooks smaller, from 0.052–0.062 mm. This species is named after Christian Gottfried Ehrenberg who was a pioneer in the study of microorganisms in the 19th century, and had a role in collecting the original type specimens.
Pachysentis gethi (Machado-Filho, 1950)
P. gethi was originally described in 1950 by Machado-Filho infesting tayra (Eira barbara) in Pará and Rio de Janeiro, Brazil but this remained the only record until it was rediscovered in 2016 infesting the wild lesser grison (Galictis cuja), also in Rio de Janeiro, with smaller measurements. The proboscis has 42 hooks without barbs arranged into six rows of four hooks followed by six rows of three hooks. The eight cement glands occur in pairs. The species is named after Dr. Geth Jansen.
Pachysentis lauroi Gomes, Amin, Olifiers, Bianchi, Souza, Barbosa & Maldonado, 2019
P. lauroi has been found infesting the South American coati (Nasua nasua) in Mato Grosso do Sul, Brazil. The proboscis has 48 barbed hooks arranged into six rows of four hooks followed by six rows of four hooks. The eight cement glands are clustered. It was found in the small intestine. The species is named after , a parasitologist who studied Brazilian Acanthocephala.
Pachysentis lenti (Machado-Filho, 1950)
P. lenti has been found infesting the white-headed marmoset (Callithrix geoffroyi) in Espírito Santo, Brazil. The proboscis has 48 hooks without barbs arranged into six rows of four hooks followed by six rows of four hooks. It is named after , a Brazilian parasitologist.
Pachysentis procumbens Meyer, 1931
P. procumbens has been found infesting the red fox (Vulpes vulpes) in Argo, Egypt. The proboscis has 90 hooks without barbs arranged into six rows of seven hooks followed by six rows of eight hooks. The species name is from the Latin procumbent, meaning "lying prostrate".
Pachysentis procyonis (Machado-Filho, 1950)
P. procyonis was found infesting the intestines of a crab-eating raccoon (Procyon cancrivorus) in Rio de Janeiro, Brazil. The proboscis has 42 hooks without barbs arranged into six rows of four hooks followed by six rows of three hooks. Distinguishing features include eight clustered cement glands and very short lemnisci that do not reach the anterior testis. The species is named after the genus of the host (Procyon) it was found infesting.
Pachysentis rugosus (Machado-Filho, 1950)
P. rugosus has been found to infest the large intestine of Azaras's capuchin (Sapajus cay) in from a dissection conducted in Rio de Janeiro, Brazil, and the tufted capuchin (Sapajus apella), also from Brazil. The proboscis is armed with 42 hooks without barbs arranged into six rows of four hooks each followed by six rows of three hooks each. This species can be identified by its clustered cement glands and long leminisci that reach the anterior testis. The species name rugosus is Latin for 'wrinkled'.
Pachysentis septemserialis (Machado-Filho, 1950)