Thuesen is a surname. Notable people with the surname include:
Marius Thuesen (1878–1941), Danish gymnast Peter J. Thuesen (born 1971), American religious scholar Peter Thuesen (sport shooter) (born 1978), Danish sport shooter Karen Thuesen Massaro (born 1944), Danish ceramist
See also Thuesen-Petersen House, is a historic pair-house in Scipio, Utah