DeStefano is an Italian surname with Greek origin. Notable people with the surname include:
Anthony DeStefano (born 1965), American author Chris DeStefano, American singer Dusty DeStefano (1917–1982), American basketball and baseball coach Frank DeStefano, medical researcher Fred DeStefano (1900–1974), American football player Joe DeStefano (born 1960), politician John M. DeStefano (died 2008), American artist John DeStefano Jr. (born 1955), American mayor Johnny DeStefano (born 1979), American political advisor Lauren DeStefano (born 1984), American author Mario Anthony DeStefano (1915–1975), American gangster Mike DeStefano (born 1966), American comedian Robert DeStefano (born 1962), American chiropractor Sam DeStefano (1909-1973), American mobster Stephen DeStefano (born 1966), American comics artist
See also Ricci v. DeStefano