The 1895 Wyoming Cowboys football team represented the University of Wyoming as an independent during the 1895 college football season. In its second season under head coach Justus F. Soule, a professor of Latin and Greek, the team compiled a perfect 1–0 record. In the program's first intercollegiate football game, the team defeated from Greeley, Colorado, by a 34–0 score.
For the second of three consecutive years, Herbert J. Brees was the team captain. Brees was a native of Laramie who went on to a career in the United States Army. He retired from the Army in 1941 with the rank of lieutenant general.
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