Skene is a surname of Scottish origin. Notable people with the surname include:

Alan Skene (1932–2001), South African rugby footballer Alexander Skene (1837–1900), Scottish gynaecologist Clyde Skene (1884–1945), Scottish footballer Debra J. Skene, South African and British chronobiologist Felicia Skene (1821–1899), Scottish author George Skene (physician) (1741–1803), Scottish physician George Skene (politician) (1749–1825), Scottish soldier and politician George Skene (provost) (1619–1708), Provost of Aberdeen James Skene (1775–1864), Scottish lawyer and friend of Sir Walter Scott James Henry Skene (1812–1886), Scottish author, traveller and British Consul at Aleppo John Skene (New Jersey official), third deputy governor of West Jersey, 1684–92 John Skene, Lord Curriehill (c.1543–1617), Scottish prosecutor, ambassador, and judge Leslie Skene (1882–1959), Scottish footballer Lilias Skene (1627–1697), Scottish Quaker preacher, prophet and poet Philip Skene (1725–1810), British Army officer and New York landowner Robert Skene (British Army officer) (1719–1787), British Army officer and MP Robert Skene (polo player) (1914–1997), American polo player Robert Skene (cricketer) (1908–1988), English cricketer William Forbes Skene (born 1809), Scottish historian and antiquary

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