Puffer is a surname. Notable people with the surname include:

Alfred Puffer (1840–1875) Brandon Puffer (born 1975), American baseball player Ethel Dench Puffer Howes (1872–1950), American psychologist and feminist Fred Puffer (c. 1871 – 1900), American track and field athlete Ruth Rice Puffer (1907–2002), American biostatistician Ted Puffer (1928–2003), American singer, voice teacher and translator William Puffer (1861–1948), Canadian politician