Wisløff is a Norwegian surname. Notable people with the surname include:
Åse Wisløff Nilssen (born 1945), Norwegian politician Carl Fredrik Wisløff (1908–2004), Norwegian theologian Jens Wisløff (1921–1998), Norwegian politician Hans Edvard Wisløff (1902–1969), 20th-century bishop of the Church of Norway Ove Wisløff (born 1954), Norwegian swimmer Ulrik Wisløff (born 1968), Norwegian physiologist