Komárek (feminine: Komárková) is a Czech surname. It is a diminutive of the word komár (meaning 'mosquito') and the surname Komár. Notable people with the surname include:
Božena Komárková (1903–1997), Czech philosopher and theologian Jaroslava Komárková (1927–2010), Czech athlete Josef Komarek (born 1992), American musician Karel Komárek (born 1969), Czech businessman Konstantin Komarek (born 1992), Austrian ice hockey player Lukáš Komárek (born 1982), Slovak ice hockey player Martin Komárek (born 1984), Czech footballer Miroslav Komárek (1924–2013), Czech linguist Valtr Komárek (1930–2013), Czech politician Vera Komarkova (1942–2005), Czech-American mountaineer
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