Zacchini is an Italian surname. Notable people with the surname include:
Edmondo Zacchini (1894–1981), American circus performer Hugo Zacchini (1898–1975), American circus performer Ildebrando Zacchini (1868–1948), Maltese-born American painter, inventor and travelling circus owner Rene A. Zacchini (1930–2010), French-American politician
See also Zacchini v. Scripps-Howard Broadcasting Co., a United States Supreme Court case