Twisleton is a surname, and may refer to:
Charlotte Twisleton née Wattell (1770-1812), English actress Edward Turner Boyd Twisleton (born 1809), British Poor Law Commissioner, son of Thomas Twisleton Francis Morphet Twisleton (1873–1917), New Zealand soldier Frederick Benjamin Twisleton (1799–1887), also Frederick Fiennes, 16th Baron Saye and Sele, son of Thomas Twisleton Sir George Twisleton, 1st Baronet (c.1605–1635) George Twisleton (1618–1667), English politician John Twisleton (c.1614–1682), English Cromwellian baronet Philip Twisleton (died 1678), English New Model Army officer Thomas Twisleton (1770/1–1824), English Archdeacon of Colombo, son of Thomas Twisleton, 13th Baron Saye and Sele Thomas Twisleton, 13th Baron Saye and Sele (c.1735–1788), British Army officer
See also Twisleton-Wykeham-Fiennes family