Acquaye is a Ghanaian surname. Notable people with the surname include:
Bianca Froese-Acquaye (born 1964), German artist, author and painter David Kpakpoe Acquaye (born 1928), Ghanaian academic and agriculturalist J. K. Acquaye (born 1940), Ghanaian academic and haematologist Nii Gyashie Bortey Acquaye (born 1999), Ghanaian footballer Saka Acquaye (1923–2007), Ghanaian artist, musician, playwright W. C. O. Acquaye-Nortey (born 1930), Ghanaian soldier and politician