A holy city is a city of special significance to a religion.

Holy City may also refer to:

The Holy City (song) Holy City, California Holy City, Devon, a hamlet in England Holy City of the Wichitas, an NRHP-listed site in the Wichita Mountains Wildlife Refuge in Oklahoma Holy City Zoo, a former comedy club in San Francisco, California

See also Four Holy Cities, the cities of Jerusalem, Hebron, Safed and Tiberias in Jewish tradition Holby City, a TV series