Bufalini is an Italian surname. Notable people with the surname include:
Francesco Antonio Bufalini, Italian draftsman, copper plate engraver and architect Giovanni Ottavio Bufalini (1709–1782), Italian cardinal Maurizio Bufalini (1787–1875), Italian physician Sauro Bufalini (1941–2012), Italian basketball player Ventura Bufalini (died 1504), Italian Roman Catholic prelate, Bishop of Terni and Città di Castello