Ayatsuji () is a family name in the Japanese language and may refer to:
Yukito Ayatsuji, a pen name for Naoyuki Uchida (born 1960), a Japanese writer.
Fictional characters: Ayase Ayatsuji, one of the supporting characters in the anime/manga Chivalry of a Failed Knight Kaito Ayatsuji, the father of Ayase Ayatsuji, one of the supporting characters in the anime/manga Chivalry of a Failed Knight Haruka Ayatsuji, one of the supporting characters in the anime/manga World Trigger Kinu Ayatsuji, one of the supporting characters in the anime/manga Sumomomo Momomo Tsukasa Ayatsuji, one of the 6 lead female characters of the anime/manga Amagami SS Yukari Ayatsuji, the older sister of Tsukasa Ayatsuji, one of the supporting characters in the anime/manga Amagami SS