Gambini is an Italian surname. Notable people with the surname include:
Pierre Gambini, French songwriter and composer Rodolfo Gambini (born 1946), Uruguayan physicist Rodolfo Gambini (painter) (1855–1928), Italian painter
See also Pier Antonio Quarantotti Gambini (1910–1965), Italian writer and journalist The Great Gambini, a 1937 American film Gambino