para-Nitroblebbistatin is a non-phototoxic, photostable myosin inhibitor with low fluorescence. Its myosin inhibitory properties are very similar to those of blebbistatin.
Myosin specificity SpeciesMyosin typeIC50Rabbit Skeletal muscle myosin S10.4 μM, 0.1 μMin press: Gyimesi et al Improved inhibitory and ADMET properties of blebbistatin derivatives indicate that blebbistatin scaffold is ideal for drug development targeting myosin-2 in Journal of Pharmacology and Experimental Therapies (2020)Dictyostelium discoideumMyosin II motor domain 2.3 μM, 9 μMHuman β-Cardiac myosin subfragment 1 13 μMChicken Heavy meromyosin fragment of skeletal muscle myosin 0.4 μMPig (left ventricle)cardiac myosin3.9 μMChicken (gizzard)smooth muscle myosin S15.6 μMHuman (expressed in Sf9 cells)non-muscle myosin 2A / B / C motor domains18 / 14 / 5 μM
Applications para-Nitroblebbistatin has been successfully used in fluorescent imaging experiments involving myosin IIA-GFP expressing live dendritic cells and synaptophysin-pHluorin expressing live neurons.
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