Zoubek (feminine Zoubková) is a Czech surname meaning literally a "little tooth". Notable people with the surname include:
Brian Zoubek (born 1988), American basketball player David Zoubek, Czech footballer Olbram Zoubek (1926–2017), Czech sculptor and designer Vladimír Zoubek (1903–1995), Czech geologist Bohuš Zoubek (1942–2017), Czech horn player and music teacher