Chamberlen is a surname of English origin. Notable people with the surname include:
Hugh Chamberlen (c.1632 – after 1720), English physician, son of Peter Chamberlen the third Hugh Chamberlen the younger (1664–1728), English physician, son of Hugh Chamberlen the elder Peter Chamberlen the elder (c.1560–1631), French barber-surgeon in England Peter Chamberlen the younger (1572–1626), English surgeon, brother of Peter Chamberlen the elder Peter Chamberlen the third (1601–1683), English physician, son of Peter Chamberlen the younger
See also Chamberlain (surname) Chamberlaine Chamberlayne (surname) Chamberlin (surname)