James Gillespie may refer to:
James Gillespie (North Carolina politician) (c. 1747–1805), American politician from North Carolina James Gillespie (minister) (1720–1791), Scottish minister and theologian James Gillespie (footballer) (1868–1932), Scottish footballer James Gillespie (philanthropist) (1726–1797), Scottish snuff and tobacco merchant James Gillespie (mariner), HM Excise officer and commander of the cutter Henry Dundas James J. Gillespie (1892–1959), American politician from Iowa James U. Gillespie (died 1981), American politician from Florida
See also Jim Gillespie (disambiguation) James G. Birney (James Gillespie Birney), American abolitionist, politician, and Liberty Party nominee for President James G. Blaine (James Gillespie Blaine), American politician and United States Secretary of State