Guidotti is an Italian surname derived from the masculine personal name Guido. Notable people with the surname include:
Galgano Guidotti (1148–1181), Italian Catholic saint Luisa Guidotti Mistrali (1932–1979), Italian Roman Catholic missionary in Zimbabwe Paolo Guidotti, also known as il Borghese (1559–1629), Italian painter, sculptor and architect Salvatore Guidotti (1836–after 1889), Italian painter Stefano Guidotti (born 1999), Swiss footballer
See also 27270 Guidotti, a main-belt asteroid named after Guido Guidotti Guidotti–Greenspan rule, for which country's reserves should equal short-term external debt Guidi