Two Brothers may refer to:
Film and television Two Brothers (1929 film), a German silent film directed by Mikhail Dubson Two Brothers (2004 film), a French-British film directed by Jean-Jacques Annaud The Two Brothers (film), a 1910 American short Western film directed by D. W. Griffith Do Bhai (disambiguation) (), various Indian films Two Brothers Pictures, a British television production company founded in 2014
Literature and folklore Tale of Two Brothers, an ancient Egyptian story The Two Brothers, a German fairy tale The Two Brothers (novel), or La Rabouilleuse, an 1842 novel by Honoré de Balzac Dois Irmãos (Two Brothers), a 2000 novel by Milton Hatoum Two Brothers (novel), a 2012 novel by Ben Elton
Music "The Twa Brothers", a traditional ballad Two Brothers (album), a 2001 album by Boxhead Ensemble
Places Two Brothers, U.S. Virgin Islands, a settlement on the island of Saint Croix Two Brothers Rocks, a rock formation in the Ionian Sea near Syracuse, Sicily
Other Two Brothers (ship), a 19th-century Nantucket whaleship Two Brothers (video game), a 2013 action role-playing game Two Brothers Brewing, an American microbrewery Two Brothers Roundhouse, on the former Chicago & Aurora Railroad, in Aurora, Illinois, U.S.
See also Deux Frères (disambiguation)