Mediothele is a genus of South American funnel-web spiders that was first described by Robert John Raven & Norman I. Platnick in 1978.
Species it contains six species, all found in Chile: Mediothele anae Ríos-Tamayo & Goloboff, 2012 – Chile Mediothele australis Raven & Platnick, 1978 (type) – Chile Mediothele lagos Ríos-Tamayo & Goloboff, 2012 – Chile Mediothele linares Ríos-Tamayo & Goloboff, 2012 – Chile Mediothele minima Ríos-Tamayo & Goloboff, 2012 – Chile Mediothele nahuelbuta Ríos-Tamayo & Goloboff, 2012 – Chile
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