The women's 100 metre freestyle S3 event at the 2016 Paralympic Games took place on 8 September 2016, at the Olympic Aquatics Stadium. Two heats were held. The swimmers with the eight fastest times advanced to the final.

Heats Heat 1 10:42 8 September 2016:

Rank Lane Name Nationality Time Notes 1 5 1:39.07 2 4 1:39.16 3 3 1:44.29 4 6 2:16.80 5 2 2:30.78 6 7 2:34.10

Heat 2 10:48 8 September 2016: Rank Lane Name Nationality Time Notes 1 4 1:39.17 2 5 1:41.82 3 3 1:45.11 4 6 2:09.03 5 7 2:18.91 6 2 2:28.57 7 1 2:59.28

Final 18:41 8 September 2016:

Rank Lane Name Nationality Time Notes 3 1:30.07 5 1:34.71 4 1:34.86 4 6 1:39.77 5 7 1:42.64 6 2 1:47.16 7 1 1:57.86 8 8 2:11.54

Notes