This is a list of characters appearing in the novel series Overlord, written by Kugane Maruyama and illustrated by so-bin, and its anime and manga adaptations.

Great Tomb of Nazarick The headquarters of the guild Ainz Ooal Gown in YGGDRASIL. After Nazarick is transported to the New World with its NPCs, who gain sentience, Ainz begins to take action to protect the tomb. However, the NPCs believe that he is planning world domination and begin to formulate plans of their own; by the time Ainz realizes their intentions, he has no choice but to go along with what they believed his plans were. This eventually leads to Nazarick becoming its own nation: the Sorcerer Kingdom.

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The leader and sole remaining member of the guild Ainz Ooal Gown. Originally, he was a salaryman named Overlord CD Drama #2. who played the VR online game YGGDRASIL, and was transported to the New World following its shutdown. He decides to make himself a legend in hopes of finding his old guildmates and changes his name from Momonga to Ainz Ooal Gown. While he inadvertently sets Nazarick on the path to world domination after a comment he made is misinterpreted, over time he grows into his role as a leader and aims to create a kingdom where humans and non-humans are equal. Due to having the form of a lich, he does not need to eat or sleep and cannot be poisoned or killed by conventional means. As well, although his emotions and empathy are suppressed, he remembers having a "heart" and so maintains some of his humanity. Because of his formerly high level in YGGDRASIL, he can easily overpower even the strongest of magic casters in the New World; despite lacking the skills of natural warriors, he compensates for it with raw strength. Over time, he learns to improve in fighting with physical weapons, but his greatest asset remains his magic. Ainz also has access to a wide variety of instant death and summoning spells which, though ineffective against fellow players, are effective against the inhabitants of the New World. He also has several items he purchased with real money back in YGGDRASIL, which give him abilities ranging from summoning high-level items from the Guild's bank to instantly casting a spell that usually has a long casting time. When traveling incognito as an adventurer, he uses the alias Momon the Dark Hero.

Floor Guardians A group of NPCs who act as the bosses of Nazarick's floors, having gained great influence over its affairs after coming to the New World and gaining sentience. They are among the most powerful beings in Nazarick, with some exceeding Ainz in stats. Their personalities and quirks come from the backstories the creators programmed into them.

A succubus and overseer of the Floor Guardians, whose beautiful appearance hides a dark personality. Shortly before YGGDRASIL was shut down, Ainz modified her to be in love with him as a joke; as a result, she falls in love with him when she arrives in the New World. Despite being a succubus, Albedo is a virgin, which she is ashamed of; however, she wishes to sleep with Ainz despite it being impossible. She despises humans, viewing them as "lower lifeforms", and also harbors hatred towards the members of the Ainz Ooal Gown Guild for leaving Ainz. While in battle, she wears a suit of armor called Hermes Trismegistus, which repels physical damage, and wields a bardiche named 3F. She also possesses the World Item Ginnungagap, which can be used to devastate large areas.

A vampire and the Guardian of Nazarick's First, Second, and Third Floors. She is said to be somewhat of a pervert and a necrophile, causing her to fall in love with Ainz and putting her at odds with Albedo. She is the most powerful Floor Guardian in terms of overall stats, specializing in both melee and magic combat. After the Slane Theocracy brainwashes her, she rebels against Nazarick until Ainz kills her and she is later resurrected. Afterwards, she blames herself for her actions despite having had no control over herself; while she later recovers from the incident, she is insecure and often belittles herself.

An energetic and extroverted girl who is the Guardian of Nazarick's Sixth Floor along with her twin brother Mare. She takes on the form of a young Dark Elf and cross-dresses as a boy. Despite their constant bickering, she is close to Shalltear, similar to the relationship between their creators. Along with Mare, Ainz treats them as if they were his children. She wields a whip and bow and can summon beasts to fight with her.

One of the Guardians of Nazarick's Sixth Floor along with his twin sister Aura. He takes on the form of a young Dark Elf and cross-dresses as a girl. Unlike Aura, he is shy and quiet, though it is implied that this personality is an act. Along with Aura, Ainz treats them as if they were his children. He specializes in Druid magic and has the ability to manipulate earth.

The Guardian of Nazarick's Seventh Floor, who is in charge of its defenses. He takes on the form of a demon who wears a business suit and glasses. He is one of Nazarick's most intelligent and evil NPCs, being willing to commit atrocities and manipulate people to further his goals. While he gets along with the other Guardians, his relationship with Sebas is strained due to their opposing natures, which were inherited from the guild members who created them, who were also often at odds. He believes that Ainz determined his schemes and only reveals parts of his true plans to appease the other Guardians' curiosity. He is known to the people of the New World as , an alternate identity he assumed to further his goals without raising suspicion, who is referred to as a powerful Demon Emperor leading armies of demons against humanity.

The Guardian of the Fifth Floor of Nazarick, who is in charge of Nazarick's security and later the Lizardmen tribes. He takes on the form of a four-armed, humanoid insect. He is noble and respects anyone with strong fighting spirit and is closest to Demiurge, who he sees as a rival and a friend. He has the power of cryokinesis and possesses several weapons.

The Guardian of Nazarick's Eight Floor of Nazarick, who speaks the Enochian language. It is Nazarick's last line of defense, with its only ability being a self-destruct skill. Despite this, it is humble and modest, believing its sole purpose is to serve the members of the guild.

The Guardian of Nazarick's Fourth Floor. It is a stone golem which, unlike the other Guardians, is not a custom NPC but an in-game bonus Ainz Ooal Gown won. As a result, it was not granted personality or free will upon coming to the New World. Due to its unique nature, it is noted to be the strongest Floor Guardian, but its strength is weakened by its inability to react on its own.

Area Guardians NPCs that serve as mini-bosses for a specific portion of a floor. Some of them serve as subordinates to the Floor Guardians, while others act outside the chain of command.

The Area Guardian of Nazarick's Treasury, who Ainz created and is as strong as a Floor Guardian. He has the ability to assume the appearance and abilities of the members of Ainz Ooal Gown, which Ainz uses to have him act as a stand-in when situations require both Ainz and Momon to be active at the same time. Because Ainz created him while going through a chuunibyou phase, he programmed him to act with exaggerated gestures and body language, which now embarrasses him. Despite not being able to spend much time together, he and Ainz share a strong bond, with Ainz allowing him to call him "father".

The Area Guardian of the Frozen Prison on Nazarick's Fifth Floor, who specializes in information gathering. She is Albedo's older sister, as they were created by the same creator, and they get along well despite being polar opposites in appearance and personality.

Nazarick's hidden super boss and Nazarick's strongest NPC. She is Albedo and Nigredo's younger sister, as they were created by the same creator, and Albedo loves her while Nigredo fears her. Ainz is initially wary of her because she might turn against them, but Albedo assures him that she is as loyal as the other NPCs in Nazarick..

Pleiades A group of NPCs dressed as maids, with the exception of Sebas, who resembles a butler. They serve as Nazarick's last line of defense before the final floor. Though they, with the exception of Sebas and Aureole, are relatively weak, their strength is on par with the legendary monsters of the New World. Despite their appearance, Nazarick's denizens see them more as like an idol group than maids.

The head butler of Nazarick and overseer of the Pleiades, who takes on the form of an elderly gentleman. Although he is not a Floor Guardian, he has abilities on par with them and specializes in unarmed combat. Unlike other NPCs, he does not hate outsiders, and like his creator, Touch Me, has a strong sense of justice. As a result, he has a habit of trying to rescue those in need, which often brings him into conflict with other NPCs and, on occasion, calling his loyalty to Ainz into question. He does not get along well with Demiurge, due to the poor relationship between their creators. In Isekai Quartet, he appears as a school staff member.

A dullahan who uses a choker to attach her head to her body and appear human. She is the second in command of the Pleiades, who look up to Aureole like a big sister, and in charge of the Sorcerer Kingdom's orphanage, which was built following the war against the Re-Estize Kingdom. She is programmed to behave like a teacher because her creator was one and holds a neutral stance towards humans, as she does not dislike them but does not want to help them either. She wields gauntlets in battle.

A werewolf who serves as the group's healer. In public, she is sociable and friendly, but in reality she is a psychopath who enjoys using her healing abilities to torture others. Ainz later assigns her to protect Carne Village.

A doppelgänger who is permanently in human form as a result of investing her levels into combat abilities; she is the strongest of the Pleiades, being a magic caster who specializes in lightning spells. She serves as Ainz's companion while under his "Momon" identity, going by the alias , during which she becomes known as "The Beautiful Princess" due to her appearance and some of the Pleiades become jealous of her due to her closeness to Ainz. She despises and looks down on humans, which often causes trouble for him.

An automaton who is silent and emotionless, and as such holds no particular dislike towards humans. She wields guns and war knives and favors long-range combat, and is the only one who knows how to unlock Nazarick's doors. She likes cute things, and is idolized by the homunculus maids.

A slime in human form who is an assassin and can absorb people and objects into her body to be stored or devoured. Being a Slime, she preys on humans and generally despises and looks down on them.

An Insectoid who disguises herself as a human by wearing a mask and changing her voice and has the ability to summon other Insectoids. As an Insectoid, she preys on humans when she is hungry, but otherwise does not consider them worthy of her attention. She holds a grudge against Evileye, who defeated and humiliated her, and seeks revenge against her. She is called the "Predator of Family" because she sometimes eats Kyouhukou's family as a snack, which has caused him to be afraid of her.

The secret seventh member of the Pleiades and becomes their leader after Sebas stepped down, as well as the Area Guardian of the Cherry Blossom Sanctuary. She is a human and the only true human in Nazarick as of the beginning of the series. Because of her dual positions, she is often unable to lead the group, with Yuri Alpha serving as leader in her stead. She is in charge of Nazarick's Teleportation Gates. Ainz seems to trust her greatly, as he entrusts her with the Staff of Ainz Ooal Gown when he leaves Nazarick.

Five Worst