Palio is the name given in Italy to an annual athletic contest, pitting the neighbourhoods of a town or the hamlets of a comune against each other.
Palio may also refer to: Fiat Palio, a supermini car by Fiat Palio (1932 film), Italian historical drama film by Alessandro Blasetti Palio (2015 film), British documentary film by Cosima Spender Palio (gastropod), a genus of sea slugs