James City may refer to the following places in the United States:
James City, North Carolina, an unincorporated area and census-designated place James City, Pennsylvania, an unincorporated community and census-designated place James City County, Virginia James City (Virginia Company), established in the Virginia Colony in 1619 James City Shire, formed in the Virginia Colony in 1634 James City Formation, a geologic formation in North Carolina
See also St. James City, Florida, an unincorporated community and census-designated place