Fanconi Anemia Opposite Strand Transcript protein is a predicted protein that in humans is encoded by the FANCD2OS gene. The name is derived from mRNA transcribed from the strand complementary to the FANCD2 gene.
Gene The gene is encoded on Chromosome 3 (human) at p25.3 on the minus strand from 10081320-10108339nt. The primary transcript is 1105nt which codes for a protein of 177 amino acid in length. The gene on the strand complementary to FANCD2 located 5' from CYCSP11 and 3’ from BRK1 and VHL genes.
mRNA There are six alternatively spliced transcripts with differences in the 5' and 3' ends as well changes in exon usage. The most common isoform is 1105 bp.
Promoter Region The promoter region predicted by the Genomatix El Dorado algorithm spans from 10108742-10108009 bp. Promoters are associated with a wide variety of tissues including B-lymphocytes, germ cells, muscle, neurons & prostate.
Regulatory Secondary structure of the 5’ UTR sequence conserved between humans and gibbon contain sequence recognized by to RBMX, Ras & Rab proteins. The sequence for the secondary structure of the 3’ UTR are recognized by EIF4B and RBMX.
Expression FANCD2OS is moderately expressed in the human brain, placenta and testes.
Evolutionary History
Protein sequence The protein from the longest transcript is 177AA in length with a mass of 20188.59kD. The protein consists of a domain of unknown function from the DUF4563 superfamily.
Orthologs Orthologs of FANCD2OS exist throughout mammals, reptiles, birds and in the cartilaginous fish Australian ghostshark. The protein sequence undergoes mutation at a rate similar to the blood protein fibrinogen.
Species Common name NCBI accession Identity similarity E-value Pongo abelii Sumatran orangutan XP_003776240.1 98.9 99.4 1.21E-133 Cricetulus griseus Chinese hamster XP_003511850.1 73.6 80.9 1.56E-95 Ovis aries Sheep XP_004018322.1 97.2 98.3 6.79E-131 Leptonychotes weddellii Weddell seal XP_006739055.1 96.6 98.3 5.13E-130 Condylura cristata Star-nosed mole XP_004692437.1 91.0 95.5 3.01E-123 Orcinus orca Killer whale XP_004286877.2 88.1 92.3 1.05E-90 Trichechus manatus latirostris Florida manatee XP_004368376.1 96.6 98.9 9.95E-131 Alligator mississippiensis American alligator XP_014457972.1 69.3 88.6 1.40E-48 Chrysemys picta bellii Painted turtle XP_005307285.1 58.3 75 2.18E-78 Gekko japonicus Schlegel's Japanese gecko XP_015261231.1 56.5 72.3 4.39E-74 Thamnophis sirtalis Common garter snake XP_013914711.1 55.9 69.5 2.75E-69 Xenopus laevis African clawed frog NP_001088778.1 55.4 71.2 1.98E-68 Xenopus tropicalis Western clawed frog NP_001090717.1 52 71.2 3.57E-65 Callorhinchus milii Australian ghostshark XP_007888744.1 39.9 56.8 5.67E-43
Paralogs FANCD2OS has no known paralogs in Homo sapiens.
References