The Lithuanian Football Cup 2000–01 was the 12th season of the Lithuanian annual football tournament. The competition started on 18 March 2000 with the first round games, and ended on 19 May 2001 with the final. The defending champions were FK Ekranas.
First round
| colspan="3" style="background:#9cc;"|18 March 2000
Quarter finals
First legs
Second legs
FK Nevėžis won 3–1 on aggregate
FK Atlantas won 2–1 on aggregate
FK Žalgiris won 5–0 on aggregate
FBK Kaunas won 13–1 on aggregate
Semifinals
First legs
Second legs
FK Žalgiris won 6–1 on aggregate
2–2 on aggregate, FK Atlantas win on away goals
Final
References