Manousakis () is a Greek surname deriving from the name Manousos. Notable people with the surname include:
Dimosthenis Manousakis (born 1981), Greek footballer Efstratios Manousakis (born 1957), Greek physicist Georgios Manousakis (born 1998), Greek footballer Manousos Manousakis (1950–2024), Greek film director, producer, writer and actor Spyridon Manousakis (born 1984), Egyptian-born Greek wrestler