The 2000–01 Russian Cup was the ninth season of the Russian football knockout tournament since the dissolution of Soviet Union.

The tournament was won by Lokomotiv Moscow who beat Anzhi Makhachkala on penalties in the final after the match finished 1–1 after extra time.

First round The first round was played on 29 March 2000, and featured Russian Football National League teams only.

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Second round The second round was played between 18 April and 2 May 2000, and featured Russian Football National League teams only.

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Third round The third round was played between 24 and 29 May 2000, and featured Russian Football National League teams only.

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Fourth round The first round was played between 9 and 26 June 2000, and featured Russian Football National League teams only.

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Fifth round Matches were played on 16 July 2000.

Round of 32

Round of 16

Quarter-finals

Semi-finals

Final

Lokomotiv Moscow:GK Ruslan NigmatullinDF Gennadiy NizhegorodovDF Dmitri SennikovDF Jacob LekgethoDF Vadim Evseev DF Igor ChugainovMF Marat IzmailovMF Vladimir MaminovMF James Obiorah MF Dmitri Loskov FW Maksim Buznikin Substitutes:MF Zaza Janashia MF Albert Sarkisyan DF Andrei Lavrik Manager: Yuri SeminAnzhi Makhachkala:GK Sergey ArmishevMF Denis Peremenin DF Syarhey YaskovichDF Michel PenséeDF Nebojša Stojković MF Elvir RahimićMF Ruslan Agalarov MF Narvik SirkhayevFW Magomed Adiyev FW Murad Ramazanov FW Predrag Ranđelović Substitutes:FW Valery Alekseyev DF Anatoli Tebloyev DF Arsen Akayev Manager: Gadzhi Gadzhiyev MATCH RULES 90 minutes. 30 minutes of extra-time if necessary. Penalty shootout if scores still level. Seven named substitutes Maximum of 3 substitutions.

References