The 2000–01 Copa Federación de España was the eighth staging of the Copa Federación de España, a knockout competition for Spanish football clubs in Segunda División B and Tercera División.

The Regional stages began in 2000, while the national tournament took place from November 2000 to April 2001.

Regional tournaments Asturias tournament

National tournament Preliminary round

Castellón and Puertollano received a bye.

Round of 16

Quarter-finals

Semifinals

Final

References 2000–2009 Copa Federación results Asturias tournament results