Ankyrin repeat domain-containing protein 24 is a protein in humans that is coded for by the ANKRD24 gene. The gene is also known as KIAA1981. The protein's function in humans is currently unknown. ANKRD24 is in the protein family that contains ankyrin-repeat domains.

Gene Locus

The gene is located on chromosome 19 at p13.3 on the forward strand. The gene is 4041 base pairs in length and contains 29 exons. The gene is neighbored by the gene SIRT6 that encodes for the Sirtuin-6 protein and the EBI3 gene that encodes for the Epstein-Barr virus induced gene 3 protein.

Expression The expression pattern of ANKRD24 is uncharacterized. Under conditions of cell growth and proliferation, the expression levels increase. In germ line tumors, glioma, and prostate cancer, the expression is elevated relative to other disease states. During development, the expression level is elevated in the blastocyst stage. In adults, there are elevated levels of expression in the placenta, stomach, kidneys, and eye relative to other tissues. However, the results of experimental gene expression profiles are inconsistent relative to ANKRD24 expression, suggesting redundancy of the gene and its protein product.

mRNA Alternative expression 13 transcription splice variants of ANKRD24 mRNA have been predicted.

Protein General features The ANKRD24 protein is 1146 amino acids in length, has a molecular weight of 124kDa, and has an isoelectric point of 4.98. The secondary structure is predicted to consist of all alpha helices and to not contain any beta strands. The tertiary structure of the protein is predicted to be a helical twist.

Composition ANKRD24 has a relatively high composition of alanine (15.0%), glutamic acid (13.5%), and leucine (11.0%) and a relatively low composition of cysteine (1.5%), phenylalanine (0.7%), tryptophan (0.2%), and tyrosine (0.8%). The protein contains positive run clusters that could be nuclear localization signals. The protein does not have any significant negative charge clusters and no significant charge patterns.

Subcellular localization The ANKRD24 protein is predicted to localize in the nucleus of cells.

Domains ANKRD24 is in the protein family that contains ankyrin-repeat domains. Ankyrin repeats are known for mediating protein-protein interactions. The protein also contains two coiled-coil regions.

Post-translational modification ANKRD24 is predicted to undergo C-mannosylation.

Interacting proteins ANKRD24 is predicted to interact with disks large homolog 4 (DLG4), eukaryotic translation elongation factor 1-alpha 1 (EEF1A1), unc-119 homolog A (UNC119), replication timing regulatory factor 1 (RIF1), protein kinase C and casein kinase substrate in neurons 1 (PACSIN1), nuclear factor NF-kappa-B p105 subunit (NFKB1), cholest-5-ene-3β,7α-diol 3β-dehydrogenase (HSD3B7), lethal giant larvae homolog 2 (L2GL2), and glucocorticoid induced 1 (GLC)CI1. No characterization of these interactions has yet to be observed.

Homology

ANKRD24 has no human paralogs. Orthologous proteins are found in other organisms. The following table represents some of the orthologs found using searches in BLAST and BLAT. However, this list is not exhaustive for the orthologs of ANKRD24 and is only meant to display the wide diversity of species for which orthologs of ANKRD24 can be found. Genus speciesCommon NameDate of Divergence (MYA)Accession # (Nucleotide)Accession # (Protein)Sequence Length (bp)Sequence Length (aa)E valueSequence Identity (%)Sequence Similarity (%)1Homo sapiensHumans0NM_133475.1NP_597732.1404111461001002Nomascus leucogenysNorthern white-cheeked gibbon19.9XM_012503370.1XP_012358824.136751165095963Otolemur garnettiiSmall-eared galago75.9XM_012804592.1XP_012660046.141811035083874Trichechus manatus latirostrisFlorida Manatee105XM_004378459.1XP_004378516.1|33541117081865Eptesicus fuscusBig brown bat97.5XM_008150610.1XP_008148832.133691122080846Ailuropoda melanoleucaGiant panda97.5XM_002928471.2XP_002928517.137291116080847Loxodonta africanaAfrican savanna elephant105XM_003421925.2XP_003421973.140491345079858Octodon degusDegu90.9XM_004632746.1XP_004632803.133811126079849Chrysochloris asiaticaCape golden mole105XM_006869037.1XP_006869099.1374712480788510Orcinus orcaKiller Whale97.5XM_004277648.2XP_004277696.1348210790778211Chelonia mydasGreen sea turle320.5XM_007072230.1XP_007072292305110169.00E-178637712Ficedula albicollisCollared flycatcher320.5XM_005060250.1XP_00506030718856212.00E-171607413Gekko JaponicusJapanese gecko320.5XM_015410656.1XP_015266142305710193.00E-175587514Python bivittatusBurmese python320.5XM_007434390XP_007434452319210631.00E-167587515Falco cherrugSaker falcon320.5XM_014277718.1XP_01413319314314760577116Stegastes partitusBicolor damselfish429.6XM_008295305.1XP_008293527.1362910952.00E-90415517Trichomonas vaginalis G3Trichomonas vaginalis2040XM_001329224.1XP_0013292597742572.00E-24345418Ciona intestinalisVase tunicate733XM_009863371.1XP_00986167331569569.00E-24324819Talaromyces stipitatusFungi1302.5XM_002486774.1XP_002486819.1321610713.00E-18325120Cryptococcus neoformansYeast1302.5 XM_012197089.1XP_012052479.19432363.00E-163251

References