TLU may mean:
Computing Tape Library Unit, an individual computer storage tape library Threshold Logical Unit in neural networks
Universities Tallinn University Texas Lutheran University "The Lords University", a jocular acronym referring to BYU, Brigham Young University
Transportation Golfo de Morrosquillo Airport (IATA airport code: TLU; ICAO airport code: SKTL) in Tolú, Sucre, Colombia Aero Toluca Internacional (ICAO airline code: TLU), see List of airline codes (A) The London Underground, the subway of London, England, UK
Other uses The Last of Us (series), a video game series and media franchise The Last of Us (2013 videogame) first game in the series Tulehu language (ISO 639 language code: tlu) St. Kitts and Nevis Trades and Labour Union Tropical Livestock Unit