Sergiyenko (; ) is a surname of Ukrainian origin. Other transliterations include Serhiyenko, Serhienko, Serhiienko, Sergeyenko, and Sergeenko.
Notable people with the surname include: Davit Sergeenko (born 1963), Georgian physician and healthcare manager Leonid Serhienko (born 1955), Ukrainian politician Mariya Sergeyenko (1891–1987), Soviet historian Ulyana Sergeenko (born 1979), Russian fashion designer Yuriy Sergiyenko (born 1965), Ukrainian athlete