The 2000–01 Liga Bet season saw Maccabi Ironi Shlomi, Hapoel Asi Gilboa, Hapoel Tira and Ironi Ofakim win their regional divisions and promoted to Liga Alef.
Second placed club, Beitar Kiryat Gat, were also promoted as the best runners-up in the South divisions, after a vacancy was created in Liga Alef South division.
At the bottom, Hapoel Bnei Kafr Yasif, Beitar Safed, Ironi I'billin (from North A division), Hapoel Arab Nujeidat, Beitar Iksal, Ironi Sayid Umm al-Fahm (from North B division), Maccabi Bnei Tira, Maccabi Kafr Qasim (from South A division) and Hapoel Rahat (from South B division) were all automatically relegated to Liga Gimel.
North A Division
North B Division
South A Division Promoted to Liga Alef: Hapoel Tira
Other league clubs: Hapoel Azor Beitar Ramat Gan Hapoel Mahane Yehuda Hapoel Hod HaSharon M.M. Giv'at Shmuel Hapoel Kafr Qasim Beitar Nes Tubruk Maccabi HaShikma Ramat Hen Shimshon Bnei Tayibe Maccabi Yehud Beitar Holon
Relegated to Liga Gimel: Maccabi Bnei Tira Maccabi Kafr Qasim
Hapoel Jaljulia and Hapoel Or Yehuda were originally set to play in the division.Israel Cups 2000/01 RSSSF Hapoel Jaljulia which finished runners-up in the previous season, were promoted to Liga Alef in order to fill a vacated spot in that league. Hapoel Or Yehuda which were relegated from Liga Alef in the previous season, withdrew from the league.
South B Division
Maccabi Jerusalem/Ma'ale Adumim and Sektzia Nes Tziona were originally set to play in the division.Israel Cups 2000/01 RSSSF Maccabi Jerusalem/Ma'ale Adumim which finished runners-up in the previous season, were promoted to Liga Alef in order to fill a vacated spot in that league. Sektzia Nes Tziona which were due to be relegated from Liga Alef in the previous season, were eventually reprieved from relegation.
References Liga Bet North, 00-01 One Liga Bet North B, 00-01 One Liga Bet South B, 00-01 One