Gladstone is a defunct electoral district that elected members to the House of Assembly, the lower house of the bicameral legislature of the then colony of South Australia.

As of 1884, its extent included the following towns - Crystal Brook, Gladstone, Georgetown, Laura and Merriton.

Members MemberPartyTermMemberPartyTerm Alfred Catt1884–1902 James Howe 1884–1891  Defence League 1891–1896  Ernest Roberts Labor 1896–1902

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